.406 Ventures is a Boston early-stage venture firm that states it backs "early-stage companies as they raise their seed and series A financings". Its three stated focus areas are healthcare, data and AI, and cybersecurity. The firm describes its team as having "over 100 years of collective experience as founders, operators, and investors", which is the firm's own claim. It operates from Independence Wharf, 470 Atlantic Avenue, Boston.
TechCrunch reports on 15 February 2024 that .406 Ventures secured $265 million for its fifth fund, and that the firm has more than $1.4 billion across its five core funds and three opportunity funds.